PMS2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91095-50, BS91095-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P54278(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:100
FC:1:50-1:100

Background: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression. Two additional homologs of the prokaryotic MutL gene, designated PMS1 and PMS2, have been identified and shown to be mutated in the germline of HNPCC patients.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PMS2 polyclonal antibody detects endogenous levels of PMS2 protein.

Bioworld Molecular Weight: 96 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PMS2 on different lysates using anti-PMS2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: SK-BR-3, ICC staining PMS2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PMS2, DNA mismatch repair gene homologue, DNA mismatch repair protein PMS2, H_DJ0042M02.9, HNPCC4, Mismatch repair endonuclease PMS2, Mismatch repair gene PMSL2, PMS 2, PMS1 protein homolog 2, PMS2, PMS2 postmeiotic segregation increased 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMS2, PMS2CL, PMSL2, Postmeiotic segregation increased, S. cerevisiae, 2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified