Nrf2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90969-50, BS90969-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16236(Human) Q60795(Mouse) O54968(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Background: The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK. It regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (Mares). A family of NF-E2- related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from its repressor protein, Keap1.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Nrf2 polyclonal antibody detects endogenous levels of Nrf2 protein.

Bioworld Molecular Weight: 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Nrf2 on mouse liver tissue lysate using anti-Nrf2 antibody at 1/500 dilution., ICC staining Nrf2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NF-E2-related factor 2, NFE2-related factor 2, , erythroid derived 2, HEBP1, like 2, NF E2 related factor 2, NF-E2-related factor 2, NF2L2, NFE2 related factor 2, NFE2-related factor 2, Nfe2l2, Nrf 2, NRF2, Nuclear factor (erythroid derived 2) like 2, Nuclear factor, nuclear factor erythroid 2 like 2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified