EIF5B Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-66431-60, E-AB-66431-120, E-AB-66431-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: EIF5B

Target Synonym: EIF5B; IF2

Research Areas: Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O60841

Background: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human EIF5B (NP_056988.3).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IF 1:50-1:200

Calculated MW: 138kDa

Observed MW: 179kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only