Phospho-Tyk2-Y1054/1055 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79399-50, BS79399-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29597

Host: Rabbit

Reactivity: Mouse

Applications: WB

All Applications: WB,1:500 - 1:1000

Background: This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Tyk2-Y1054/1055 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by IFN-α at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Bioworld Molecular Weight: 134KDa

Specificity: Phosphorylated

Alternative Name: TYK2; IMD35; JTK1

Immunogen: A synthetic phosphorylated peptide around Y1054 & Y1055 of human Tyk2(NP_003322.3).

Conjugate: Unconjugated

Modification: Phosphorylated