Phospho-AURKA-T288 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79389-50, BS79389-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14965

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa and HT-29 cells, using Phospho-AURKA-T288 antibody at 1:1000 dilution. HeLa cells were treated by Hydroxyurea for 20 hours. HeLa cells were treated by Nocodazole for 20 hours. HT-29 cells were treated by Hydroxyurea for 20 hours or treated by Nocodazole for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 48kDa

Specificity: Phosphorylated

Alternative Name: AURKA; AIK; ARK1; AURA; BTAK; PPP1R47; STK15; STK6; STK7

Immunogen: A synthetic phosphorylated peptide around T288 of human AURKA(NP_003591.2).

Conjugate: Unconjugated

Modification: Phosphorylated