Phospho-STK3/MST2-T384 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74195-50, BS74195-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13188

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-STK3/MST2-T384 antibody at 1:1000 dilution. HeLa cells were treated by Calyculin A at 37℃ for 30 minutes. NIH/3T3 cells were treated by Calyculin A at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 54kDa

Specificity: Phosphorylated

Alternative Name: KRS1; MST2; STK3

Immunogen: A synthetic phosphorylated peptide around T384 of human STK3/MST2(NP_006272.2).

Conjugate: Unconjugated

Modification: Phosphorylated