QuicKey Pro Mouse IgG3 (Immunoglobulin G3) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-OSEL-M0009-24, E-OSEL-M0009-48, E-OSEL-M0009-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: IgG3

UNIProt ID: IgG3

Target Synonyms: IgG3

Research Areas: Immunology

Target Species: Mouse

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IgG3 concentrations in serum, plasma.

Tested Sample Types: Serum, Plasma

Type: Sandwich

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 0.14 ng/mL

Detection Range: 0.31-20 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG3 are added to the micro ELISA plate wells. Mouse IgG3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgG3. The concentration of Mouse IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Mouse IgG3 in samples. No significant cross-reactivity or interference between Mouse IgG3 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃, 6 months

Shipping: ice bag