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Glass-backed Prep TLC Plates

Miles Scientific Analtech Brand Silica Gel GF Prep TLC Plates - Glass-backed - 500µm thickness - 20x20cm - 25 plates per box

Product Number: P02012

Weight: 12.10 LBS

• G - (Gypsum) Calcium Sulfate Hemihydrate binder
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

Preparative TLC plates have adsorbent layers thicker than the 250 micron layers normally used for analytical separations. Thus, preparative plates can be used for separation of larger quantities of materials.

The loading capacity for a given degree of sample component resolution increases roughly as the square root of the increased adsorbent layer thickness. In preparative TLC, materials to be separated are often applied as long streaks rather than spots. After development, specific compounds may be recovered by scraping the adsorbent layers from the plate in the region of interest and eluting the separated material from the adsorbent using a strong solvent.

• Thicker layers for higher capacity
• Full range of plate sizes
• Calcium sulfate binder-soft later for easy scraping

Analtech Brand Silica Gel GF TLC UNIPLATES™

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Calcium sulfate a.k.a. gypsum (inorganic)
• Hardness: 20-40

Acceptable Solvents:

All typical chromatography solvents (20% water max)

Visualization Techniques:

Compatible with essentially all visualization reagents, even sulfuric acid charring. Spraying reagent is suggested; layer is incompatible with aqueous dipping technique.

General Description:

Considered to be the original “Classic” TLC plate. Gypsum (calcium sulfate) binder produces softer layer for easy scraping and removal of spots or bands for further analysis. This is the best choice when preparative work is needed using thicker coatings to isolate larger quantity of material. Because the binder is inorganic, it is compatible with aggressive visualization techniques such as sulfuric acid charring. Limited water tolerance due to slight solubility of calcium sulfate in water precludes dipping procedure but is applicable with spray techniques.

Silica Gel G & GF UNIPLATES are the most widely used silica gel TLC plates. No organic substances are added to these products, thus making them compatible with sulfuric acid charring visualization.These plates are commonly scraped from the glass backing to isolate separated components for other analytical procedures. They are compatible with all organic solvents, however, they are not recommended for use in systems containing greater than 20% water because of the solubility of the gypsum binder in aqueous solutions. (For water tolerance see the GHL & GHLF series of plates.)

The "G" stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4·1/2H2O), a refined form of gypsum, has been included in the adsorption formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate.

The "F" indicates the presence of a short wave UV indicator that is fluorescent at 254 nm.

• Fluorescent indicator has green fluorescence at 254 nm
• Soft Layers with gypsum binder enable easy scraping of bands from plate
• G & GF layers contain no organic materials 

Preparative TLC plates have adsorbent layers thicker than the 250m microns. Thus, preparative plates can be used for separation of larger quantities of materials. The loading capacity for a given degree of sample component resolution increases roughly as the square root of the adsorbent layer thickness. In preparative TLC, materials to be separated are often applied as long streaks rather than spots. After development, specific compounds may be recovered by scraping the adsorbent layers from the plate in the region of interest and eluting the separated material from the adsorbent using a strong solvent.

• Thicker layers for higher capacity 
• Full range of plate sizes
• Prep-scored option for convenient zone location
• Available with preadsorbent sample loading zone
• Calcium sulfate binder-soft later for easy scraping